Papers on "Growing and Enumerating E.coli Colonies" and similar term paper topics

Paper #103272 :: Growing and Enumerating E.coli Colonies
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This paper concerns experiments within the field of microbiology and discusses growing and enumerating E.coli colonies.

Written in 2007; 1,025 words; 3 sources; MLA; $ 36.95

Paper Summary:

The writer points out that the purpose of this laboratory was to acquire fundamental knowledge and experience in microbiological procedures, specifically in growing bacterial colonies on a nutrient medium under controlled conditions. In this case, Escherichia coli (E. coli) bacteria were grown on nutrient agar, contamination was minimized by keeping the surroundings sterile, and all agar plates were incubated. The writer notes that the first part involved making five serial dilutions from a bacterial suspension in order to pinpoint colony numbers. The second experiment compared two methods of spreading bacteria across agar plates using streaks. The goal was to isolate single colonies. Lastly, the third portion of the laboratory involved replicating plates from a master plate in order to isolate strains of mutant bacteria onto different nutrient mediums and determine bacterial auxotrophy to these nutrients.

Outline:
Introduction
Materials and Methods
Results
Discussion
References

From the Paper:

"In the streaking experiment, method B proved to be much more effective for isolating single colonies. The different streaking methods may have contributed to the morphological differences between the E. coli on the two plates. There were evident differences in the size and colour. Since method B was dominated by spaced out single colonies, the darker colour and larger size can be attributed to more nutrients being available to each colony, and more room (surface area) for growth. For the same reason, the more crowded groups of bacteria on the method A plate were a lighter colour and generally smaller. Aside from these two differences, the E. coli shared great resemblance, and based on morphology alone, should not be classified as different species. Nonetheless, the only way to determine whether or not two sets of visibly different bacteria are genetically different is to actually observe their DNA."

Tags: microbiological bacteria contamination

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