| Papers [1-14] of 100 :: [Page 1 of 8] | | Go to page : 1 2 3 4 5 6 7 8 —> | Search results on "GOLGI PRION PROTEIN PATHOGENESIS": |
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Golgi in Prion Protein Pathogenesis, 2005.
An NIH-style paper presenting information on prions, pathogenesis and potential treatments.
1,950 words (approx. 7.8 pages), 11 sources, APA, £ 44.95
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Abstract
The paper is written in NIH standard format style. It details a completely novel, potential experiment in determining prion protein pathogenesis by use of the Golgi. The paper outlines the specific aims, the materials and methods, expectations, and future work in a logical order. The purpose of this research is to determine whether or not the Golgi Apparatus is instrumental in facilitating the distribution of infectious prion proteins from infected neurons. It is hypothesized that constitutive exocytosis from the Trans end of the Golgi Apparatus is a vector through which infectious prions exit an infected neuron. It is also hypothesized that chemical inhibition of exocytosis may decrease formation of pathogenic PrPSc from healthy PrPc.
The paper includes figures.
From the Paper
"Steps toward understanding the nature of these dreaded pathogens are extremely important. Many lives may depend on the development of a treatment, since it is unknown how many people may be carrying PrPSc. The death inflicted by prion illnesses is a slow, excruciating, unimaginably frightening ordeal. In addition, infectious prions are extremely resilient, able to withstand temperatures that carbonize meat, sterilizing doses of UV radiation, and standard autoclaving (4). The pathogenic PrPSc protein poses health risks to not only those who consume and work with beef, but also to physicians, nurses, and technicians who may be exposed to potentially-contaminated neurological tissue."
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Prions and Prion Diseases, 2001.
A discussion of the various aspects of prions and prion diseases.
1,307 words (approx. 5.2 pages), 4 sources, £ 31.95
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Abstract
This paper examines the novel mechanism of disease transmittal in prions as well as the diseases themselves (mad cow disease and Creutzfeld-Jacobs disease). Functions of normal prions are also discussed. The paper also examines how prions are devoid of nucleic acids, and thus carry no information other than their tertiary structure.
From the Paper
"Knockout mice survived up to their 70th week, and then they began to display signs common with brain disease, which included a loss of motor coordination. Curiously, the symptoms shown by these knockout mice were similar to those found in many cases of prion diseases."
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Prions: Proteinaceous Infectious Particles, 2004.
A discussion of prions, the proteins that are typically expressed in brain tissue and that may exist in a normal or abnormal shape, and prion diseases.
1,725 words (approx. 6.9 pages), 11 sources, APA, £ 39.95
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Abstract
This paper explains what prions are and how degenerative brain diseases result from abnormal prions. The paper discusses professor Stanley Prusiner's discovery of prions and pediatrician Carleton Gajdusek's contributions to this discovery, as well as the prion diseases themselves. Additionally, the paper explains the infectious nature of the prion protein and explains the progression of prion diseases and tests used to diagnosis the disease.
From the Paper
"Recent cases of Mad Cow Disease have focused the public attention on prion diseases and the small proteins that are believed to cause them. The scientific community has been slow to recognize this mechanism of disease, since prion-caused encephalopathies can demonstrate diverse symptoms, and share characteristics with other disorders, such as dementia."
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Protein Folding, 2004.
An analysis of the process of protein folding by which a protein assumes its functional shape or conformation.
1,828 words (approx. 7.3 pages), 14 sources, MLA, £ 41.95
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Abstract
This paper discusses the importance of proteins and protein folding to the human body. The paper explains that, during the different stages of protein folding that include primary, secondary, tertiary and sometimes-quaternary structures, the protein becomes susceptible to incorrect folding by binding with other molecules. The paper explores these ?misfoldings?, which may cause diseases such as Alzheimer?s disease, cystic fibrosis, and many cancers. The paper states that researchers continue to try and comprehend the reasons and methods of protein ?misfolding? to further our knowledge of the diseases that are caused by it.
From the Paper
"Proteins are the biochemical molecules that make up cells, organs and organisms. (1) Like the larger structures, proteins also put themselves together, in a process termed "folding." Protein folding is the process by which a protein assumes its functional shape or conformation. All protein molecules are simple unbranched chains of amino acids, but it is by coiling into a specific three-dimensional shape that they are able to perform their biological function. The proteins fold into either alpha or beta helices with the assistance of chaperon proteins. (1)"
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The CYP2D Protein in the Human Brain, 2005.
An analysis of the characterization of the CYP2D protein from human brain cerebellum.
14,400 words (approx. 57.6 pages), 0 sources, APA, £ 178.95
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Abstract
The CYP2D protein in the human brain is expressed as CYP2D6 in liver and is known to metabolize a variety of drugs such as psychoactive, antihypertensives, and environmental toxins. Every person has a unique rate of metabolism. Metabolic rates in children are particularly susceptible to environmental toxicants which affect the way in which their systems absorb, distribute, metabolize and excrete chemicals. To date, knowledge in characterization of CYP proteins has been limited to immunoblotting, RT-PCR, immunohistochemistry and so forth; therefore, this paper investigates:
1) What kind of CYP2D protein is present in brain with modern proteomic tools;
2) Whether, CYP2D6 is the same as liver CYP2D6; and
3) If CYP2D7 is present, can CYP2D6 polymorphism be found in the brain?
To answer these questions, this study uses a one-step method of isolation of protein by immunoprecipitation followed by its identification using 2D-blots, nanospray LCMS, immunoblots and immunohistochemistry.
Table of Contents
Introduction
Problem Statement
Hypothesis
Rationale
Literature Review
Results
Discussion
Conclusion
References
Appendix
From the Paper
"The most promising area of pharmacogenetic research today may be in providing patients with customized treatments for specific cancer types. For instance, Christensen points out that Herceptin and Gleevec are two drugs that affect only cancerous tissues expressing certain genes. In the case of Herceptin, it binds to a protein that stimulates rapid tumor growth; this substance has been found to be present in excess in approximately 33 percent of breast cancers. Prior to prescribing the drug to their patients, doctors can now test how many copies of the gene for HER2/neu, which encodes this protein, are present or measure concentrations of the protein itself. Patients who do not have the excess HER2/neu protein or its gene would therefore not receive the drug. Gleevec takes advantage of a particular enzyme that is mutated in a few rare cancers, such as chronic myeloid leukemia. When Gleevec blocks the mutated enzyme, growth of the cancer cells stops; however, the drug has minimal effects on healthy dividing cells (Christensen, 2002)."
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Proteins, 2005.
This paper discusses proteins and their role with athletics.
2,920 words (approx. 11.7 pages), 5 sources, MLA, £ 61.95
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Abstract
This paper explains that proteins are called the building blocks of life because they are essential to the proper functioning of every organism known to man. It indicates that they are used for support, storage, transport of other substances, signaling from one part of the organism to another, movement and defense against foreign substances. The author points out that eating an appropriate amount of protein is important to everyone because ingesting too much or too little can both have adverse affects on the rest of the body. The paper reports that nutritionists have identified several categories of athletes who may require greater levels of protein to reach peak performance: Endurance athletes, athletes performing intense strength training programs, teenage athletes with growth as well as exercise requirements, exercisers and athletes following a calorie-restricted weight loss program.
From the Paper
"Exercise intensity has been shown to alter the rates by which amino acids are oxidized-or converted to energy-by the body. This has not been found to be a linear progression of intensity to oxidation, but rather an exponential relationship. Therefore, an exercise routine that is just slightly more intense than another routine may trigger the oxidation of a significantly larger amount of amino acid. This fact needs to be accounted for when considering the protein needs of an athlete with a particularly intense type of workout."
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Proteins, Carbohydrates and Lipids, 2004.
An overview of proteins, carbohydrates and lipids, detailing their structure and functions in the body.
1,350 words (approx. 5.4 pages), 2 sources, APA, £ 33.95
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Abstract
This paper gives an introduction to the chemical substances of life: proteins, carbohydrates and lipids. It describes how proteins are formed, their primary, secondary, tertiary, and quaternary structure, and gives examples of protein use in the human body. The paper also describes mono- and poly-saccharides, their structure, reactions, and functions in the human body. Lastly it describes lipids and fatty acids and their functions in the human body.
From the Paper
"Proteins contain the elements carbon, hydrogen, oxygen and nitrogen and some may also contain sulfur. The building blocks of proteins are amino acids. There are twenty essential amino acids found in cells and some proteins contain special amino acids which are derived from the essential twenty by a change in their constituents. They contain an amino group at one end (NH) and a hydroxyl group at the other (OH) and in between there is a carbon atom with one hydrogen and.
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Isolation and Purification of Muscle Proteins, 2008.
This paper discusses the isolation and purification of actin and myosin in skeletal myofibrils.
1,791 words (approx. 7.2 pages), 7 sources, APA, £ 40.95
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Abstract
The writer explains that in the set of experiments discussed in the paper, myosin and actomyosin were isolated and gradually purified from rabbit muscle myofibrils. The two proteins were initially precipitated together out of solution, in order to remove any impurities. Then, the two proteins could be separated from each other, by taking advantage of differential solubility. The writer notes that after each time they were re-suspended or redissolved, the solutions were centrifuged in order to further improve purity. Any solution containing the contractile proteins or ATP were kept on ice during the experiments, and stored in a freezer at all other times. The writer discusses that using the Bradford protein assay and the SDS-PAGE, the concentrations and weights of the purified proteins in the final solutions were measured. For all protein samples, no appreciable concentration of protein could be detected after diluting the samples by 100 times. However, the other two dilutions yielded normal results.
Outline:
Abstract
Introduction
Materials and Methods
Results
Discussion
From the Paper
"The head of the myosin is the part which interacts with the other molecules. Without ATP, the myosin head is bound tightly to the actin, called the rigor state. However, ATP weakens this interaction by opening a cleft in the myosin head and disrupting the actin-binding site. When the myosin head is free of actin, it hydrolyzes ATP to ADP. This causes a conformational change in the head that moves it to a new position, closer to the (+) end of the actin filament, (away from the origin site) where it reattaches. The dissociation of the phosphate from ATP also causes a conformational change, this time in the myosin head. As a result, myosin returns to its original state (the rigor state). Because myosin is still bound to actin, this conformational change exerts a force that causes myosin to move the actin filament. This is the power stroke. Essentially, contraction is a series of these power strokes by the actomyosin complex, the myosin head is moving along the actin filament through constant detachment and reattachment."
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C-Reactive Proteins, 2004.
An analysis of C-reactive proteins as a marker for heart disease.
2,033 words (approx. 8.1 pages), 5 sources, MLA, £ 45.95
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Abstract
This paper defines c-reactive protein (CRP), an acute phase plasma protein that responds to inflammatory conditions by binding to damaged cells and activating complement, increasing proportionately to inflammatory stimuli. It explains that CRP as an indicator of heart disease risk or onset of myocardial infarction could be valuable in terms of early detection and potential future use of CRP inhibitors for therapeutic value. This paper also shows that evaluation, on an individual basis, for the serum testing of CRP should be based on findings from the physical exam and medical history to determine the true nature of CRP elevations, with positive implications for disease prevention and treatment.
From the Paper
"Cardiovascular disease is the leading cause of death in the Western World, killing approximately 17 million people per year. It can take the form of high blood pressure, coronary heart disease, peripheral vascular disease, stroke, or rheumatic heart disease. Risk factors include old age, smoking, obesity, heredity, race, physical inactivity, hyperlipidemia, poor diet, high blood pressure, increased alcohol consumption, diabetes, and stress. The medical history would consider these risk factors, with a physical exam noting the patient?s condition relative to the presented risk factors. Combined, these elements could warrant further testing to diagnostically determine the patient?s risk or development of heart disease, its stage, and characterization."
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High Protein Diets, 2001.
This paper focuses on the new craze of high protein diets. The detrimental effect on critical body systems is discussed in depth.
1,850 words (approx. 7.4 pages), 6 sources, £ 42.95
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Abstract
A paper on the new craze of high-protein, low-carbohydrate, low- fat diets and their effects on the body. The paper focuses on the deleterious effects of a high protein diets for those with pre- existing medical conditions, diabetes, renal dysfunction and heart disease.
From the Paper
"It appears to me that Americans are becoming the most overweight population on Earth. We are gaining weight faster than ever. We have all heard of the fly-by-night diets and weight loss programs guaranteeing overnight results. It appears there is a propensity of those who appear physically fit to do well in society and no doubt that in our world exists inescapable superficiality. The will of those trying to lose: fervent, the price could be your life. The range is vast, from those trying to shed those very last few pounds for the bathing suit season to those who are medically morbidly obese. We spend enormous amounts of dollars on these purported miracles, only to succumb to the reality of medical quackery: no results. ?In 1990, Americans spent over 33 billion on weight- loss products and services.? (Miles, Petrie & Steel, 2000, p.254) Sadly, those who are overweight will try anything and everything to lose those pounds, and in doing so, may be causing harm."
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G Protein-Linked Receptors, 2004.
An analysis of G protein-linked receptors in communication between cells.
3,973 words (approx. 15.9 pages), 16 sources, MLA, £ 76.95
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Abstract
This paper analyzes the fundamentals of the large and complex body of information already collected and still in progress on the subject of G protein-linked receptors. The paper highlights the basic nature of G protein-linked signaling and explains how physiological regulation occurs through particular mechanisms.
From the Paper
"Cells in multi-cellular organisms, like animals, need to communicate among themselves in directing and regulating growth, development and organization (Altruis Biomedical Network 2003). Such communication modes include secreting chemicals that signal to distant cells, display cell surface chemicals that influence other cells in direct physical contact, and directly through porous cellular points called gap junctions (Altruis Biomedical Network). Endocrine signaling demonstrates the first mode, wherein hormones are secreted in the bloodstream to distant target cells. Paracrine signaling illustrates the second mode, wherein local chemical mediators are secreted and act only on cells in the proximate environment. And synaptic signaling exhibits the third mode, wherein molecules are released by vesicles at those junctions called synapses. The molecules are neurotransmitters that spread out and act only on the postsynaptic target cell (Altruis Biomedical Network). Protein receptor molecules that are on or within the target cells bind to the hormone, paracrine or neurotransmitter and a response results, depending on the speed and selectivity of the delivered signal."
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Amino Acids, Carbohydrates and Protein Synthesis, 2002.
This paper explores the proper timing of amino acids and carbohydrates in regard to protein synthesis.
3,175 words (approx. 12.7 pages), 13 sources, MLA, £ 65.95
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Abstract
This paper attempts to explore the proper timing of amino acids and carbohydrates in regard to protein synthesis. It does this by examining the causes of catabolism and what happens during this process, by looking at the building blocks and the types of proteins involved in the process and by studying the function of insulin and how it functions in relation to the intake of carbohydrates.
From the Paper
"The field of Biochemistry which ?studies the structure, synthesis and degradation of large molecules found in living cells, their metabolic regulation, and their expression (Spencer, 1997)? is known as molecular biology. These large molecules, or macromolecules, include DNA, RNA, proteins and carbohydrates, and their complexes."
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Soy Protein and Bone Health in Women, 2004.
A study on the possible bone health benefits for women from soy protein.
1,131 words (approx. 4.5 pages), 5 sources, APA, £ 28.95
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Abstract
This paper reviews studies that support the theory that high soy diets have a beneficial effect on the relative density or mineral content of bones, as well as studies that have shown no connection between bone health and high soy diets. The paper concludes that, while evidence is inconclusive regarding the role that soy may play in women's health, the general nutritional benefits of soy protein are, nonetheless, well-established.
From the Paper
"Soy, a protein-rich legume, has been prominently featured in the traditional cuisines of Far Eastern cultures for thousands of years. In addition to its high protein content, soy also contains the other two macronutrients, carbohydrates and fats, as well as many vitamins, minerals and other micronutrients such as calcium, folic acid, and iron. Dietary sources of soy protein include soybeans, tofu, beans curd, tempeh, miso, and soymilk. Since it represents a vegetable source of high quality complete protein, containing all of the essential amino acids, soy is particularly valued by vegetarians, who must derive all their dietary protein from vegetables rather than from animal products.(DGPL)"
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The Role of Proteins in Mitosis, 2006.
A paper on the role of microtubule-associated proteins in mitosis.
1,112 words (approx. 4.4 pages), 4 sources, APA, £ 27.95
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Abstract
This paper describes the model that was used to study the role of microtubule-associated proteins in mitosis. The paper explains that microtubules play a significant role in a number of cellular processes, including mitosis, cell motility, and intracellular vesicle transport. It also points out that the dynamic behavior of microtubules plays a significant part both in cell cycle actions and in cell segregation.
From the Paper
"Kinesins and dyneins play significant roles throughout cell dissection. By means of RNA intrusion (RNAi) to reduce individual or groupings of motors along with immunofluorescence and time-lapse microscopy, the mitotic roles of cytoplasmic dynein and all 25 kinesins in Drosophila S2 cells are examined. It is shown that four kinesins are concerned in bipolar spindle assembly, four kinesins are involved in metaphase chromosome alignment, dynein plays a role in the metaphase-to-anaphase transition, and one kinesin is needed for cytokinesis. efficient redundancy and alternative pathways for completing mitosis were observed for many single RNAi knockdowns, and failure to complete mitosis was observed for only three kinesins. As an example, inhibition of two microtubule-depolymerizing kinesins originally formed monopolar spindles with unusually long microtubules, but cells ultimately formed bipolar spindles by an acentrosomal pole-focusing mechanism. From our phenotypic data, we construct a model for the distinct roles of molecular motors during mitosis in a single metazoan cell kind. ."
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