Analyte Prolactin

Paper Summary:

This paper examines how human prolactin is a single chain polypeptide hormone composed of four antiparallel alpha helices with three intrachain disulphide loops between residues 4-11, 58-174 and 191-199 and how post translationally it is modified by cleavage, glycosylation, phosphorylation, and dimerisation to give multiple forms. It looks at how its function in females is to stimulate and maintain lactation and how the main control over prolactin secretion from the anterior pituitary is by hypothalamic factors.

Outline
Description of Analyte and Structure
Purpose of Analysis and Main Practical Problems
Preparation of Samples For Analysis
Storage of Samples
Description and Principle Method Used For Detection
Method Performance
References

From the Paper:

"Two biological assays that are involved in the detection and measurement of prolactin are the pigeon crop-sac assay and the in vitro isolated mammary gland assay. The pigeon crop-sac assay is the classical bioassay for prolactin. It is based on the crop-sac response of incubating pigeons, which is believed to be the equivalent of mammalian lactation. The crop-sac response is thought to be stimulated by prolactin. The original assay method consists of intramuscular injection of the test solution every day, for four to six days. The crop-sacs are then excised at necropsy and the stimulated areas are weighed."

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